Dermatophytosis of pigs by Trichophyton mentagrophytes

We describe the isolation of Trichophyton mentagrophytes in two family-operated farms where the animals were suffering skin ailments characterized by a swelling and a reddening of the back and flanks.This condition affected 2 and 5% respectively of the animals on the farms, the younger ones being more frequently affected.     

Effect of visible light on carotenogenesis in arthroconidiating Trichophyton mentagrophytes

The effect of visible light on carotenoid content in the dermatophyte Trichophyton mentagrophytes ATCC 26323 was investigated. The fungus T. mentagrophytes accumulated several carotenoids when arthroconidiated on Sabouraud glucose agar at 37°C. When this fungus was irradiated with moderate fluence rates of white light, the resultant arthroconidia contained considerably less carotenoids in comparison with dark controls although growth and arthroconidiation of this fungus were not at all affected by visible light. The reduction of carotenoid content in arthroconidia was due primarily to blue light, although red light caused a slight decrease in pigmentation. The suppressive effect of visible light on pigmentation was fluence rate dependent. Carotenoid accumulation in arthroconidia was inversely and exponentially related to the fluence rate of light. Carotenoid formation in arthroconidiating T. mentagrophytes was neither photoinducible nor photostimulative. An analysis of isolated carotenoids revealed that visible light caused a quantitative reduction in pigmentation, and no single carotenoid was selectively decreased.Non-standard abbreviations PI pigmentation index - r coefficient of correlation     

须癣毛癣菌致儿童脓癣1例

报道由须癣毛癣菌引起的儿童头部脓癣1例.患儿为4岁幼女,因头皮部丘疹1个月,脓肿4d就诊.内服青霉素V钾无效.取断发镜检查见发外真菌孢子,培养鉴定为须癣毛癣菌,细菌培养为棒状杆菌.经内服和外用特比萘芬抗真菌,静脉输入头孢噻肟钠联合克林霉素及万古霉素抗细菌治疗,12d后脓肿减轻,细菌培养阴转,但真菌培养仍阳性,继续抗真菌治疗2个月后皮损消退,真菌检查阴性.     

难辨认癣1例

36岁女性患者,右小腿丘疹红斑、脓疱、渗液伴瘙痒5个月。查体见:右小腿可见大小不等的丘疹红斑,伴有糜烂渗液。真菌荧光镜检:阳性,真菌培养:须癣毛癣菌,诊断:难辨认癣。口服伊曲康唑分散片0.1 g,2次/日,外用酮康唑乳膏2次/日,治疗2周后,皮损明显好转,治疗1个月后,右小腿皮肤基本恢复正常,目前随访中。     

260例浅部真菌病及致病菌种分析

目的了解新疆乌鲁木齐市浅部真菌病发病情况及其病原菌的菌种分布。方法对2004年2月至2010年08月在新疆医科大学第一附属医院皮肤科门诊就诊,临床拟诊为头癣、甲癣、体股癣、手足癣的1 308例患者取病发、皮屑、甲屑行真菌镜检、培养。培养阳性标本在形态学鉴定的基础上行核糖体DNA(rDNA)ITS区序列测定确切鉴定菌种。使用SPSS 17.0统计软件对于结果进行统计分析。结果真菌镜检培养均为阳性患者260例,头癣培养阳性率最高,占33.1%,其次为体股癣占28.8%、甲癣占21.5%、手足癣占16.5%;菌种鉴定:红色毛癣菌为最常见的致病菌(28.5%),其次为须癣毛癣菌(22.3%)、犬小孢子菌(18.8%)、断发毛癣菌(10.0%)。犬小孢子菌为头癣最主要致病菌(16.2%)。统计学分析显示:不同性别、族别间体股癣、手足癣发病率有统计学意义(P<0.05),均好发于男性,汉族发病率高于维吾尔族。不同年龄头癣、甲癣、体股癣、手足癣发病率差异也均有统计学意义(P<0.05),甲癣、体股癣、手足癣好发于中青年,头癣好发于儿童。结论乌鲁木齐市浅部真菌病主要致病真菌是红色毛癣菌。头癣是本地区最主要的儿童浅部真菌病,主要病原菌为犬小孢子菌。     

广州地区甲真菌病致病真菌的变迁趋势研究

目的为了解广州地区甲真菌病的致病菌种分布情况.方法笔者采用真菌培养法对临床症状典型或镜检阳性的甲真菌病病甲进行培养.结果分离出致病真菌618株,其中皮肤癣菌417株,占67.5%,酵母菌149株,占23.8%,霉菌54株,占8.7%.结论广州地区的甲真菌病的致病菌除皮肤癣菌外,酵母菌,霉菌也占一定的比例,近几年酵母菌感染有上升趋势.     

The Effect of Fungal Morphology on Ligninolytic Enzyme Production by a Recently Isolated Wood-Degrading Fungus Trichophyton Rubrum LSK-27

The morphology and ligninolytic enzyme production of a recently isolated wood-degrading fungus Trichophyton rubrum LSK-27 was investigated. In submerged cultures, the organism appeared to be an efficient manganese peroxidase (MnP) producer. When grown in baffled and unbaffled shake flasks with three different working volume/total volume ratios (WV/TV 10, 25 and 50%), the organism displayed notable morphological differences, with variations in pellet shape and size. Cultivation in baffled flasks with 25% WV/TV resulted in higher MnP and also laccase production as well as an earlier appearance of these enzymes in culture broth. However, oxygen limitation conditions inhibited MnP and laccase production and resulted in considerable changes in the morphology of this fungus.     

须癣毛癣菌所致脓癣病发的扫描和透射电镜观察及菌体外酶活性分析：2例报告

目的报道2例由须癣毛癣菌引起的脓癣,并对病发做扫描和透射电镜观察及菌体外分泌酶活性测定。方法例1为6岁女孩,表现为头顶部炎性肿块、脓血伴脱发;例2为1岁8个月男孩,表现为头部红斑,无脓肿及脱发。家中分别饲养宠物狗和兔。病发真菌镜检均阳性后做真菌培养、生化鉴定及分子生物学鉴定。对2例患儿病发用扫描和透射电镜进行超微结构观察。对2株分离的菌株分别进行细胞外分泌酶测定。结果2株菌均鉴定为万博节皮菌（须癣毛癣菌的有性期）。扫描电镜见例1的病发受累毛干表面大量炎性组织碎屑、红细胞、脓细胞及上皮细胞;透射电镜在毛皮质内发现菌体。扫描电镜见例2的受累毛干表面真菌孢子及炎性渗出物;透射电镜见毛发内外菌体。2株菌的外分泌酶主要为碱性磷酸酶、白氨酸芳胺酶、β-葡萄糖甙酶、N-乙酰-葡萄糖胺酶、α-甘露糖甙酶。经口服伊曲康唑胶囊等综合治疗后2例均痊愈,未见不良反应。结论确诊2例须癣毛癣菌所致脓癣,伊曲康唑胶囊等药物联合治疗有效。